The aim of this study was to evaluate the potential use of sisal and coir fibers in combination with Eucalyptus particles for the production of particleboard. The particleboards were produced in three layers. The first and third layers (face) were made with small Eucalyptus particles. The second layer (core) was made with big Eucalyptus particles in combination with coir or sisal fibers. The particleboards were prepared with the substitution on Eucalyptus wood for sisal and coir fibers in the particleboards core, in doses of 0, 10, and 20%, relative to the total mass of particles. The particleboards were characterized by mechanical, physical and thermal properties. The results were not satisfactory for particleboards with sisal. However, for coir particleboards the physical-mechanical properties were very similar to those particleboards produced only with Eucalyptus. This work demonstrates the potential use of the coir that is commonly disposed in landfills on the Brazilian beaches.